Bio-Rad's pGLO Bacterial Transformation Kit is the classic kit for teaching the central dogma and the basics of genetic engineering. In this bacterial transformation lab activity, students use the pGLO plasmid to transform bacteria to express green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria, which causes the bacteria to …Avery–MacLeod–McCarty experiment. The Avery–MacLeod–McCarty experiment was an experimental demonstration by Oswald Avery, Colin MacLeod, and Maclyn McCarty that, in 1944, reported that DNA is the substance that causes bacterial transformation, in an era when it had been widely believed that it was proteins … Ingredients should include organic plant proteins like pea protein, hemp protein, and seed proteins. However, plant proteins can lack critical nutrients like BCAAs (Branched-Chain Amino Acids). Whey Protein Powder: Milk is made from two dairy-based proteins; casein and whey. The best whey protein powder is low in lactose and has all 9 essential ... Nighttime Protein Shake Supplement Facts. Curb Nighttime Hunger, Sleep Better, and Reduce Stress All with 1 Shake Stop Nighttime Hunger to Support Weight Loss Nighttime snacking can sabotage your weight loss with unneeded calories. And guess what? 25% of your daily calories (approx. 900) are consumed after dinner.Transformation Protein contains 30 grams of protein and just 210 calories per serving. It is a synergistic blend of egg white, plant and collagen proteins. It is 100% pure and natural with no dairy, no casein, no soy or whey.Discussion. Natural transformation is a mechanism of horizontal gene transfer and has fundamental roles in bacterial genome evolution ( Ambur et al., 2016 ). The ComEC protein mediates the critical step of ssDNA membrane translocation, and as such it serves as an essential component of the NT machinery.The protein transformation is one part of a broader remodeling of the food system. As value pools form around new technologies and processes that help address such critical issues as taste, health, and cost, the need for some long-standing processes, such as animal slaughtering and meat packing, will decline.Transformation's delicious Super Greens + Berries now comes in single serving packs of 10. Get a full day’s serving of superfood vegetables & fruits with an unbeatable taste to support your immune system, boost natural energy levels. In stock | …Abstract. Recent developments in deep learning, coupled with an increasing number of sequenced proteins, have led to a breakthrough in life science applications, in particular in protein property prediction. There is hope that deep learning can close the gap between the number of sequenced proteins and …Protein localization is a suggestion for its function. Therefore, we conducted subcellular localization analysis using protoplasts of Populus simonii × P. nigra to study the function of the PsnMLP328 gene preliminarily. ... PEG concentration, and transformation time [20, 21]. The protein subcellular localization is fundamental for ...A protein coding gene was successfully cloned in an expression vector that was confirmed by sequencing. This construct containing the gene was transformed into a protein expression host like BL21 (DE3) and colonies were obtained. After growing a large-scale culture and harvesting the cells, it was found …Complete with MCT Oil and L-leucine, Transformation Protein accelerates fat burn and optimizes body composition changes as quickly as poss. FREE SHIPPING & RETURNS | MONEY-BACK GUARANTEE +1 (800) 916-4237 Shop All. Our Story. BLOG. RECIPES. HELP. Account; Search; Shop All Our Story ...Transformation Protein is a cutting-edge dietary supplement formulated to support muscle growth and body transformation. It combines premium whey protein isolate, casein, and plant-based proteins, providing a comprehensive amino acid profile. These proteins play essential roles in muscle repair, recovery, and development.Epicotyls transformed with the citrus GRF–GIF chimera showed a 4.7-fold increase in regeneration frequency compared to those transformed with the empty vector control (Supplementary Fig. 6a).2. Chicken Parm with Sweet Potato Fries. This baked-not-fried version of the recipe makes it much healthier than the traditional versions. Not only is chicken high in protein, but it's also a lean option. Utilizing sweet potatoes as a side are a more nutritious option to pair with than regular potatoes would.Transformation Protein Powder Review | Is this the ultimate protein powder? - YouTube. 0:00 / 13:54. •. Intro. Transformation Protein Powder Review | Is this the ultimate … Transformation Protein. Transformation Protein is a cutting-edge dietary supplement formulated to support muscle growth and body transformation. It combines premium whey protein isolate, casein, and plant-based proteins, providing a comprehensive amino acid profile. These proteins play essential roles in muscle repair, recovery, and development. Nov 20, 2020 · With 30 grams of protein per serving, transformation protein has the perfect blend of collagen peptide, egg white, and plant proteins to achieve the ideal amino-acid profile. Including 3g of the most important muscle-building amino acid L-leucine, required for optimal muscle gain and minimal muscle loss. No other plant protein meets this ... Protein is an acid-forming food, and to sustain muscle you need an adequate amount of protein daily. So, if your body is too acidic, it’s not only hard to maintain lean muscle, but also more difficult to achieve your goals. Taking Super Greens daily can help, plus it tastes great and provides the vitamin equivalent of a …Transformation Protein 241 N Broadway, Suite 502 Milwaukee, WI 53202. Join The VIP List. Stay up to date on exclusive promotions, fitness tips, delicious recipes and more! Sign Up. [email protected]. Transformation Protein 241 N. Broadway, Suite 502 Milwaukee WI 53202Forecasts for the global digital transformation market are $1 trillion by 2025, at a staggering CAGR of 16.5% from 2020. This transition has expanded marketing efforts' reach …3. Addictive Chunky Monkey Bulking Protein Smoothie. The addition of nut butter make this a protein-packed all-star smoothie for quicker recovery and bulking up. There are actually over 50g total protein in just one shake if you use this recipe. Plus you get a boost of healthy fats from nut butter.Transformation Protein is a dairy-free, keto-friendly, soy-free, non-GMO protein powder that helps you build muscle, lose weight, rejuvenate skin and hair, and support brain and gut health. It contains egg white, plant protein, MCT oil, collagen, and other ingredients to …Transformation and selection of bacteria are key steps in DNA cloning. DNA cloning is the process of making many copies of a specific piece of DNA, such as a gene. The copies are often made in bacteria. In a typical cloning experiment, researchers first insert a piece of DNA, such as a gene, into a circular piece of DNA called a …Through a strategic expansion of its relationship of more than 20 years, Accenture has been selected by Adobe to co-develop industry-specific solutions using Adobe Firefly, …YES, our protein super blend has zero added sugar. It is sweetened with all natural and plant-based stevia and monk fruit. How many servings are in bag of Transformation Protein? There are 18 full servings (2 scoops for 12-16oz shake) or 36 half servings (1 scoop for 8-12oz shake) per bag of Transformation Total …Mar 1, 2023 · Transformation's Super Greens + Mixed Berries can help to support your immunity, detoxify the body, improve overall digestion, reduce inflammation and add natural energy to recover faster, all while providing the equivalent of a full day’s serving of fruits and vegetables. We recommend drinking Super Greens first thing in the morning with 8 ... Precise protein assemblies not only constitute a series of living machineries but also provide an advanced class of biomaterials. Previously, we developed the inducing ligand strategy to generate various fixed protein assemblies, without the formation of noncovalent interactions between proteins. Here, we …Protein–protein interaction (PPI) plays a fundamental role in numerous cellular functional progresses [1–5].PPI sites refer to the interfacial residues of proteins that are involved in these interactions, and the identification of PPI sites is of utmost importance for unraveling the mysteries of cell processes and promoting the development of new …3. Addictive Chunky Monkey Bulking Protein Smoothie. The addition of nut butter make this a protein-packed all-star smoothie for quicker recovery and bulking up. There are actually over 50g total protein in just one shake if you use this recipe. Plus you get a boost of healthy fats from nut butter.The most common cause is an imbalance in dietary macronutrients – your balance of protein, carbohydrates and fats is wrecking your metabolism. Metabolic Meltdown If your annual physical reveals weight gain along with high blood pressure, elevated blood sugar, or elevated blood lipids, these are the clinical signs of The Metabolic …Transformation Protein is perfect to add if you're looking to bulk up or build more muscle, especially those with a fast metabolism! Transformation Protein has your back! It's already got 30 grams of protein all you need to do is add some more carbs and fats to the mix and you're set! This easy recipe is so simple just add one full …Transformer neural networks have revolutionized structural biology with the ability to predict protein structures at unprecedented high accuracy. Here, we report the predictive modeling performance of the state-of-the-art protein structure prediction methods built on transformers for 69 protein targets from the recently concluded 15th Critical ...Protein localization is a suggestion for its function. Therefore, we conducted subcellular localization analysis using protoplasts of Populus simonii × P. nigra to study the function of the PsnMLP328 gene preliminarily. ... PEG concentration, and transformation time [20, 21]. The protein subcellular localization is fundamental for ...A commonly used classroom experiment to demonstrate genetic transformation employs the pGLO plasmid and Escherichia coli as the host. A detailed structure of the plasmid is shown in Figure 1.The 5371 bp plasmid contains a gene for a green fluorescent protein (GFP), which originally came from a bioluminescent jellyfish …Transformation Protein contains 30 grams of protein and just 210 calories per serving. It is a synergistic blend of egg white, plant and collagen proteins. It is 100% pure and natural with no dairy, no casein, no soy or whey.Coagulation is the culinary process where liquid protein components in a mixture transform into a more solid, often gel-like state. Similar to gelatinization, which applies to the thickening of starches, coagulation is the thickening of proteins. Like denaturation, heat, acid or mechanical action also initiates coagulation.2 Scoops Transformation Protein Powder Chocolate ; 2 Tbsp Peanut butter Powder; 10-12 Ounces non-dairy alternative; STEP BY STEP. 1. Add all liquid ingredients first followed by dry. 2. Shake until smooth. TAGS. Chocolate Protein, Peanut Butter, ...Group Size: For 10 lab groupsTime Required: Set Up - 50 minutes / Incubation - overnight / Transformation - 15 minutesKit Includes: Instructions, BactoBeads™ E. coli GFP Host, supercoiled pFluoroGreen™ plasmid DNA, ampicillin, IPTG, CaCl2, Growth Additive, ReadyPour™ Luria Broth Agar (sterile), Luria Broth Medium for Recovery (sterile), petri …Transformation Protein Super Blend | Egg White, Collagen Peptides, and Plant Protein | 15 Billion CFU Probiotics | Digestive Enzymes | MCT Oil | BCAAs | Low Carb Shake …Major steps of the Agrobacterium tumefaciens-mediated plant transformation process. (1) Attachment of A. tumefaciens to the plant cells. (2) Sensing plant signals by A. tumefaciens and regulation of virulence genes in bacteria following transduction of the sensed signals. (3) Generation and transport of T …Precise protein assemblies not only constitute a series of living machineries but also provide an advanced class of biomaterials. Previously, we developed the inducing ligand strategy to generate various fixed protein assemblies, without the formation of noncovalent interactions between proteins. Here, we …Comparison of cell-to-cell transformation with artificial transformation. Strain names: CAG, CAG18439; HB, HB101; and MC, MC4100. Cell-to-cell transformation (A) was performed with three combinations including donor cells harbouring pHSG299. ... CAG18439 is a protein or peptide, we performed the following three experiments. As …The DNA of interest, or the protein coded for by the DNA, can then be isolated and purified. When is transformation used? Bacterial transformation is used: To make multiple copies of DNA, called DNA cloning. To make large amounts of specific human proteins, for example, human insulin, which can be used to treat people …Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. ... Bacteria co-transformed with recombinant proteins and chaperones cloned in independent plasmids are suitable for …A commonly used classroom experiment to demonstrate genetic transformation employs the pGLO plasmid and Escherichia coli as the host. A detailed structure of the plasmid is shown in Figure 1.The 5371 bp plasmid contains a gene for a green fluorescent protein (GFP), which originally came from a bioluminescent jellyfish …1. In a large bowl, sift together the flour, baking powder, salt and stevia. Make a well in the center and pour in the almond milk, egg and melted butter; mix until smooth. 2. Heat a lightly oiled griddle or frying pan over medium-high heat. Pour or scoop the batter onto the griddle, using approximately 1/4 cup for each pancake.Nighttime Protein Shake Supplement Facts. Curb Nighttime Hunger, Sleep Better, and Reduce Stress All with 1 Shake Stop Nighttime Hunger to Support Weight Loss Nighttime snacking can sabotage your weight loss with unneeded calories. And guess what? 25% of your daily calories (approx. 900) are consumed after dinner. Banana Protein Smoothie. 38. RECIPE BY. Teresa Parker. PREP TIME. 5 minutes. Simple recipe! all you need is Transformation protein, almond milk, and a Banana. Bananas tend to be higher in carbohydrates especially if you let them over-ripen! If you're counting your carb intake just use half a banana! Transformation Protein contains 30 grams of protein and just 210 calories per serving. It is a synergistic blend of egg white, plant and collagen proteins. It is 100% pure and natural with no dairy, no casein, no soy or whey.At the theoretical level, the steps needed for obtaining a recombinant protein are pretty straightforward. You take your gene of interest, clone it in whatever expression vector you have at your disposal, transform it into the host of choice, induce and then, the protein is ready for purification and characterization.With the TRANSFORMATION PROTEIN App, you will have access to workout programs designed specifically to help you reach your fitness and health goals! You can follow & track your workouts, your nutrition, your lifestyle habits, measurements, and results–all with the help of your coach. FEATURES: - Access …2. Chicken Parm with Sweet Potato Fries. This baked-not-fried version of the recipe makes it much healthier than the traditional versions. Not only is chicken high in protein, but it's also a lean option. Utilizing sweet potatoes as a side are a more nutritious option to pair with than regular potatoes would.Epicotyls transformed with the citrus GRF–GIF chimera showed a 4.7-fold increase in regeneration frequency compared to those transformed with the empty vector control (Supplementary Fig. 6a).TP53INP2 repression is part of an adaptive mechanism aimed at preserving muscle mass under conditions in which insulin action is deficient. Enables ubiquitin binding activity. Acts upstream of or within several processes, including macroautophagy; osteoblast differentiation; and ubiquitin-dependent protein catabolic process.Perform Whey Protein Isolate is designed to help support lean-body goals by providing 25 grams of high-quality protein to help your muscles recover after training and build back stronger. Each serving is only 120 calories and just 1-2 grams of net carbs, making it a great protein powder to fuel your post-workout recovery and …The first strategy is based on chloroplast transformation, followed by gene expression and translation in chloroplasts. This has proven to be a powerful approach for the production of proteins at high levels. The second approach is based on nuclear transformation, followed by post-translational import of proteins from the cytosol into …Super Greens help to support your immunity, detoxify the body, reduce inflammation and improve overall digestion. Providing the equivalent of a full day’s serving of fruits and vegetables, Super Greens are 100% Non-GMO, vegan, gluten-free, soy-free, whey-free and dairy-free. Nourish your body and feel amazing with Super Greens.Transformation's delicious Super Greens + Berries now comes in single serving packs of 10. Get a full day’s serving of superfood vegetables & fruits with an unbeatable taste to support your immune system, boost natural energy levels. In stock | …To perform log transformation of the observations for each protein we take our data and use select to exlude the columns of character vectors and the pipe the output to log2() and use the pipe again to create a data frame. Then we use bind_cols to bind the first two columns of dat_pvals followed by dat_log and the last column of …The primary challenge in sequence-based protein design is the vast space of possible sequences. A small protein of 30 residues (mean length in eukaryotes ≈ 472, ref. 1) translates into a total ...Specifically, Transformation Protein, although a more complete protein than some of the other types, has a similar shelf life to other brands. With 30 grams of protein per serving, transformation protein has the perfect blend of collagen peptide, egg white, and plant proteins to achieve the ideal amino-acid profile.Forecasts for the global digital transformation market are $1 trillion by 2025, at a staggering CAGR of 16.5% from 2020. This transition has expanded marketing efforts' reach …Amyloid formation is a transition between the dilute solution phase of a protein and a solid β-sheet-rich aggregated phase that occurs through nucleation-and-growth processes. Uncontrolled ...Massive breakthrough in Alzheimer’s treatment could ‘transform lives’. A jab designed to remove toxic proteins from the brain before they develop into dementia is …Transformation Protein is a cutting-edge dietary supplement formulated to support muscle growth and body transformation. It combines premium whey protein isolate, casein, and …CARB IQ is a unique combination of the patented version of Dihydroberberine called GlucoVantage® along with chromium picolinate and cinnamon bark extract. Studies have shown that chromium and cinnamon have synergistic effects when paired together. This, along with Dihydroberberine, combines to make a powerhouse …Mar 24, 2021 · Food for Thought: The Protein Transformation. By Benjamin Morach , Björn Witte , Decker Walker , Elfrun von Koeller , Friederike Grosse-Holz , Jürgen Rogg , Michael Brigl , Nico Dehnert , Przemek Obloj , Sedef Koktenturk, and Ulrik Schulze. The world’s consumers love animal-based protein—so much so that in 2020 they ate 574 million metric ... Transformation Protein 241 N Broadway, Suite 502 Milwaukee, WI 53202. Join The VIP List. Stay up to date on exclusive promotions, fitness tips, delicious recipes and more! Sign Up. [email protected]. Transformation Protein 241 N. Broadway, Suite 502 Milwaukee WI 53202Revolutionary Protein Superblend Formula. Body strength is maximized with 30Gs of amino optimized egg white and plant proteins, and collagen peptides. Gut health is supported with a synergistic combination of pre & probiotics, and sun fiber. Brain power gets a boost with MCT oil. We are the #1 protein designed to fuel total …Jan 24, 2019 · Foods to eat at this time can include vegetables of any kind, two pieces of fruit, low fat cottage cheese with carrots, oatmeal and whey protein, or whole meal sandwiches with chicken or beef. Eat about 30-minutes after training for replenish energy stores. Eat all of the six daily meals outlined in this plan. Mar 24, 2021 · Food for Thought: The Protein Transformation. By Benjamin Morach , Björn Witte , Decker Walker , Elfrun von Koeller , Friederike Grosse-Holz , Jürgen Rogg , Michael Brigl , Nico Dehnert , Przemek Obloj , Sedef Koktenturk, and Ulrik Schulze. The world’s consumers love animal-based protein—so much so that in 2020 they ate 574 million metric ... Transformation Protein. Transformation Protein is a cutting-edge dietary supplement formulated to support muscle growth and body transformation. It combines premium whey protein isolate, casein, and plant-based proteins, providing a comprehensive amino acid profile. These proteins play essential roles in muscle repair, recovery, and development. After induction, when the T7 RNA polymerase is produced, most of the cellular protein synthesis machinery will be devoted to producing the target protein. On occasion, low-level expression of T7 polymerase within these strains leads to expression of the recombinant protein and may slow or prevent growth of the transformed bacteria.Mar 13, 2020 · Whey Protein For Weight Loss. Whey is not only a powerful muscle-builder, it can also support weight loss. In fact, a study from the USDA reported that men and women drinking two whey protein shakes per day for 12 weeks (without dieting or exercising!) lost 5 pounds of body fat, dropped an inch from their waists and even gained muscle.[4] Those ... A commonly used classroom experiment to demonstrate genetic transformation employs the pGLO plasmid and Escherichia coli as the host. A detailed structure of the plasmid is shown in Figure 1.The 5371 bp plasmid contains a gene for a green fluorescent protein (GFP), which originally came from a bioluminescent jellyfish …Discussion. Natural transformation is a mechanism of horizontal gene transfer and has fundamental roles in bacterial genome evolution ( Ambur et al., 2016 ). The ComEC protein mediates the critical step of ssDNA membrane translocation, and as such it serves as an essential component of the NT machinery.Florida State University. Genetic transformation is the process by which an organism acquires and expresses a new gene. Genetic engineering is the directed transfer of a gene, or piece of DNA, into a cell (typically a bacteria). Typically the intent is to force the cell to express (produce) the protein that the newly …30G Multi-Protein Superblend - Perfectly amino optimized protein amount for optimal lean muscle growth. Transformation Protein contains the optimal amount and ratio of all 9 essential protein amino acids for optimal body composition transformation. Including the clinically proven amount of the most …Jan 24, 2019 · Foods to eat at this time can include vegetables of any kind, two pieces of fruit, low fat cottage cheese with carrots, oatmeal and whey protein, or whole meal sandwiches with chicken or beef. Eat about 30-minutes after training for replenish energy stores. Eat all of the six daily meals outlined in this plan. The Science Behind Protein Transformation. Proteins are made up of amino acids, which are the building blocks of our bodies. When we consume protein, our bodies break it down into these amino acids, which are then used to build and repair tissues. Additionally, consuming protein can help us feel fuller for longer periods, which may aid in ... Buy SPR BODY Transformation Chocolate Protein Powder, Super Greens Superfood Green Juice Powder and Performance Insulated Shaker Bottle on Amazon.com FREE SHIPPING on qualified ordersBreakfast. Blueberry, yogurt & ricotta “pancakes”. Simply add greek yogurt and ricotta to your pancake mix to beef up the protein content. Lunch. Turkey chili. Dinner. Chicken fried cauliflower rice. Sub regular rice for brown, purple, or cauliflower rice for a healthy, low-carb substitute.Nighttime Protein Shake Supplement Facts. Curb Nighttime Hunger, Sleep Better, and Reduce Stress All with 1 Shake Stop Nighttime Hunger to Support Weight Loss Nighttime snacking can sabotage …4LifeTransform Bar is a high-protein nutrition bar with high-quality proteins like whey, soy, hydrolyzed collagen, and milk protein isolates to support your body transformation goals, satisfy between meal cravings, and promote lean muscle mass and strength. It’s the only protein bar in the world powered by 4Life …
The protein transformation is one part of a broader remodeling of the food system. As value pools form around new technologies and processes that help address such critical issues as taste, health, and cost, the need for some long-standing processes, such as animal slaughtering and meat packing, will decline.. The keegan at mission glen
Protein localization is a suggestion for its function. Therefore, we conducted subcellular localization analysis using protoplasts of Populus simonii × P. nigra to study the function of the PsnMLP328 gene preliminarily. ... PEG concentration, and transformation time [20, 21]. The protein subcellular localization is fundamental for ...Protein is an acid-forming food, and to sustain muscle you need an adequate amount of protein daily. So, if your body is too acidic, it’s not only hard to maintain lean muscle, but also more difficult to achieve your goals. Taking Super Greens daily can help, plus it tastes great and provides the vitamin equivalent of a …Transform supplements are inspired by you and backed by science. Founded on quality and performance, we work with the industry’s top scientists and formulators to develop clinically-effective products that … Transformation Protein Powder can help women over 40 find the right balance of protein and other key nutrients their bodies need. Transformation Protein contains a premium blend of egg white, collagen peptide and plant proteins along with all nine essential amino acids, MCT Oil, probiotics, and digestive enzymes. Transformation Protein is a dairy-free, keto-friendly, soy-free, non-GMO protein powder that helps you build muscle, lose weight, rejuvenate skin and hair, and support brain and gut health. It contains egg white, plant protein, MCT oil, collagen, and other ingredients to …Red Fluorescent Proteins for Gene Expression and Protein Localization Studies in Streptococcus pneumoniae and Efficient Transformation with DNA Assembled via the Gibson Assembly Method. ... Indeed, transformation with unpurified Gibson mixes readily yielded correct transformants. Thus, this direct transformation of assembly …Stay up to date on exclusive promotions, fitness tips, delicious recipes and more! LINDSEY MATHEWS. Certified Strength & Conditioning Specialist, Certified Sports Nutritionist, and Precision Nutrition Certified with 15+ years in the weight loss industry. 9,124. Certified reviews. Powered by YOTPO. Want 10% off Your First Order? Sign Up Below! Home of the #1 rated meal replacement shake. Programs, supplements, and resources ... Transformation products can be purchased directly via our websites at https://transformationprotein.com and https://transformationgreens.com. We also have our products available on Amazon. If you need help placing a manual order or have a question about one of our products, you can call our team directly at 800-916-4237.Buy SPR BODY Transformation Chocolate Protein Powder, Super Greens Superfood Green Juice Powder and Performance Insulated Shaker Bottle on Amazon.com FREE SHIPPING on qualified ordersApr 19, 2019 · April 19, 2019. Transformation's Total Body Protein super blend consists of egg white, collagen and plant proteins, complete with all 9 essential amino acids to promote lean muscle growth. For best results we recommend taking directly after your workout (within 30 minutes) for post-workout recovery or 1-2 hours prior to your workout for fuel ... Complete with MCT Oil and L-leucine, Transformation Protein accelerates fat burn and optimizes body composition changes as quickly as poss. FREE SHIPPING & RETURNS | MONEY-BACK GUARANTEE +1 (800) 916-4237 Shop All. Our Story. BLOG. RECIPES. HELP. Account; Search; Shop All Our Story ... Transformation Protein The only Multi-Protein Superblend that gets you total body results. FREE SHIPPING & RETURNS | MONEY-BACK GUARANTEE +1 (800) 916-4237 .